Brilliant Cresyl Blue stain selects largest oocytes with highest mitochondrial activity, maturation-promoting factor activity and embryo developmental competence in prepubertal sheep
نویسندگان
چکیده
منابع مشابه
Developmental competence of prepubertal goat oocytes selected with brilliant cresyl blue and matured with cysteamine supplementation.
The aim of this study was to assess the effect of oocyte selection using the brilliant cresyl blue (BCB) test plus the addition of cysteamine to the in vitro maturation (IVM) medium to improve the in vitro embryo development of prepubertal goat oocytes. The oocytes were exposed to 26 microM BCB and classified according to their cytoplasm coloration: BCB+ (oocytes with blue cytoplasm) and BCB- (...
متن کاملSelection of Ovine Oocytes by Brilliant Cresyl Blue Staining
Sheep oocytes derived from the ovaries collected from the slaughterhouse are often used for research on in vitro embryo production, animal cloning, transgenesis, embryonic stem cells, and other embryo biotechnology aspects. Improving the in vitro culture efficiency of oocytes can provide more materials for similar studies. Generally, determination of oocyte quality is mostly based on the layers...
متن کاملDevelopmental competence of bovine oocytes selected based on follicle size and using the brilliant cresyl blue (BCB) test
BACKGROUND Many studies reported that follicle size has an essential role in developmental potential of oocytes. Also, the brilliant cresyl blue (BCB) test is one of the most important criteria in selection of more competent oocytes. OBJECTIVE Selection of developmentally competent bovine oocytes. MATERIALS AND METHODS A total of 1730 bovine cumulus oocyte complexes (COCs) were recovered fr...
متن کاملthe effect of e-64 on the developmental competence of sheep cocs during in vitro maturation
in the present study, the effect of e-64 at different concentrations (0.5, 1, 5 and 10 µm) added to (1) the ivm medium on oocyte nuclear maturation and developmental competence of ovine oocytes, and (2) to the ivc medium on embryonic development of ovine embryos were investigated.
developmental competence of bovine oocytes selected based on follicle size and using the brilliant cresyl blue (bcb) test
background: many studies reported that follicle size has an essential role in developmental potential of oocytes. also, the brilliant cresyl blue (bcb) test is one of the most important criteria in selection of more competent oocytes. objective: selection of developmentally competent bovine oocytes. materials and methods: a total of 1730 bovine cumulus oocyte complexes (cocs) were recovered fro...
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ژورنال
عنوان ژورنال: REPRODUCTION
سال: 2011
ISSN: 1470-1626,1741-7899
DOI: 10.1530/rep-10-0528